10% Neutral Buffered Formalin
Cat No.: DCP-NBF4X
Size: 500 mL
Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
10% Neutral Buffered Formalin (NBF) is the gold standard fixative for histopathology laboratories worldwide, offering superior tissue preservation for microscopic examination. This ready-to-use solution contains approximately 4% formaldehyde buffered to a neutral pH, creating the optimal environment for tissue fixation while preventing common artifacts. Our premium formulation ensures consistent, reliable results for both routine diagnostic procedures and advanced research applications.
As a non-coagulant and additive fixative, 10% NBF creates cross-links between aldehydes and tissue proteins, forming a protective gel-like matrix that preserves cellular constituents in their original relationships. This process effectively prevents autolysis and putrefaction while maintaining critical microscopic details. Formaldehyde penetrates tissue rapidly, though the complete fixation process occurs gradually as cross-links form, ensuring thorough preservation throughout the specimen.
Applications:
- Routine histological tissue fixation in diagnostic settings.
- Preparation of samples for light microscopy.
- Immunohistochemistry procedures (with appropriate antigen retrieval).
- Long-term preservation of museum specimens.
- Research applications requiring consistent morphological preservation.
Advantages:
- Excellent preservation of nuclear and cytoplasmic details
- Compatible with most staining techniques and molecular procedures
- Suitable for both short-term fixation and long-term storage
- Well-suited for high-throughput laboratory environments
- Permits restoration of natural color in museum specimens
Composition:
| Ingredients |
Concentration |
| Formaldehyde (40%) |
100 mL/L |
| Sodium phosphate, monobasic (NaH₂PO₄·H₂O) |
4.0 g/L |
| Sodium phosphate, dibasic (Na₂HPO₄) |
6.5 g/L |
Usage Instructions:
- Ensure specimen containers are properly labeled before fixation.
- Place tissue specimens in the fixative solution immediately after collection when possible.
- Maintain a fixative-to-tissue ratio of at least 10:1 for optimal results.
- For routine histology, fix tissues for 6-24 hours (larger specimens may require longer fixation times).
- After fixation, process tissues according to standard laboratory protocols.
Quality Control:
- Appearance: Clear solution
- pH: 7.4
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at room temperature away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
Safety Precautions:
CAUTION: Contains formaldehyde, a potential carcinogen. Proper handling procedures must be followed:
- Use in well-ventilated areas or preferably under a fume hood.
- Wear appropriate personal protective equipment including chemical-resistant gloves, laboratory coat, and eye protection.
- Avoid contact with skin, eyes, and mucous membranes.
- Do not ingest or inhale vapors.
- Keep away from incompatible materials and sources of ignition.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
