0.625% Glutaraldehyde in 0.1 M Phosphate Buffer
Cat No.: DCP-GAP0.6X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane Twice.
Description:
0.625% Glutaraldehyde in 0.1 M Phosphate Buffer is a specialized fixative solution designed for high-precision applications in biological research, particularly electron microscopy and tissue preservation. This pre-mixed formulation combines the crosslinking efficacy of glutaraldehyde with the pH stability of a phosphate buffer system, offering researchers a reliable and convenient tool for structural studies. The solution is optimized to balance tissue penetration and fixation quality, making it suitable for delicate specimens requiring minimal artifactual distortion. The glutaraldehyde concentration is calibrated to provide sufficient crosslinking of proteins and lipids while maintaining compatibility with downstream staining or immunohistochemical protocols. This buffer system stabilizes the pH during fixation, critical for preserving ultrastructural details and preventing acid hydrolysis of sensitive cellular components.
Applications:
- Electron Microscopy: The primary application of this fixative is in transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Its low glutaraldehyde concentration (0.625%) allows deeper penetration into tissues compared to higher concentrations (e.g., 2.5–5%), reducing the risk of over-fixation artifacts while stabilizing organelles, membranes, and cytoskeletal structures. The phosphate buffer maintains isotonicity, minimizing osmotic damage during immersion fixation.
- Histochemistry and Immunology: The formulation is compatible with post-fixation processing for enzyme histochemistry and immunogold labeling. The absence of potassium in the phosphate buffer (in some variants) prevents interference with potassium-sensitive antibodies, making it ideal for neurological and immunological studies
- Tissue Preservation: Used for immersion fixation of small biopsies (≤1 mm³) and perfusion fixation of organs, the solution preserves tissue architecture for light microscopy, immunohistochemistry, and long-term storage. Its moderate crosslinking density retains antigenicity better than higher glutaraldehyde concentrations, enabling subsequent antibody binding
Advantages:
- Lower Cytotoxicity: The 0.625% concentration reduces tissue hardening compared to higher glutaraldehyde concentrations, facilitating sectioning for ultrastructural analysis.
- Reduced Discoloration: Unlike stronger glutaraldehyde solutions, this formulation minimizes yellowing of specimens and agar substrates, a common issue with unneutralized aldehydes.
Composition:
| Ingredients |
Concentration |
| Glutaraldehyde |
0.625% |
| Phosphate Buffer |
0.1M |
Safety and Handling:
Hazard Classification
-
Harmful if inhaled, with risks of respiratory irritation and sensitization.
-
Causes skin and eye irritation.
- May induce allergic skin reactions.
First Aid
Protective Measures
-
Use in a fume hood or well-ventilated area.
-
Wear nitrile gloves, safety goggles, and a lab coat.
-
Avoid skin contact and aerosol formation during pipetting.
Quality Control:
- Appearance: Clear solution
- pH: 7.4
- Sterility: Filtered 0.1 micron Twice and 0.04 micron Twice.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at 4°C away from bright light (amber) or Store at -20°C away from bright light for long term storage
- Shelf life is 6 months at 4°C. Shelf life is 18 months in -20°C unopened
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
