LoSeraTM Nutrient Mixture F-12 Ham With Sodium bicarbonate Without L-Glutamine (Liquid requiring reduced serum supplementation)

Product#: HM-RSL010
$68.08
HM-RSL010
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LoSera™ Nutrient Mixture F-12 Ham
With Sodium bicarbonate
Without L-Glutamine
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-RSL010

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

RSL010 is LoSera™ Nutrient Mixture F-12 Ham medium with sodium bicarbonate. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  44.100   Biotin  0.0073
Copper sulphate pentahydrate  0.0025   Choline chloride  13.960
Ferric sulphate heptahydrate  0.834   D-Ca-Pantothenate  0.480
Magnesium sulphate anhydrous  57.650   Folic acid  1.320
Potassium chloride  223.600   Nicotinamide  0.037
Sodium bicarbonate  1176.000   Pyridoxine hydrochloride  0.062
Sodium chloride  7599.000   Riboflavin 0.038
Sodium phosphate dibasic anhydrous  142.040   Thiamine hydrochloride  0.340
Zinc sulphate heptahydrate  0.863   Vitamin B12  1.360
AMINO ACIDS     i-Inositol  18.000
Glycine 7.510   OTHERS  
L-Alanine  8.910   D-Glucose  1801.600
L-Arginine hydrochloride  210.700   Growth Supplement mix  Proprietary
L-Asparagine anhydrous  15.010   Hypoxanthine sodium salt  4.770
L-Aspartic acid  13.300   Linoleic acid  0.084
L-Cystine dihydrochloride  35.120   Lipoic acid  0.210
L-Glutamic acid  14.700   Phenol red sodium salt  1.240
L-Glutamine  146.000   Putrescine dihydrochloride  0.161
L-Histidine hydrochloride monohydrate  20.960   Sodium pyruvate  110.100
L-Isoleucine  3.940   Thymidine  0.730
L-Leucine  13.100      
L-Lysine hydrochloride  36.500      
L-Methionine  4.480      
L-Phenylalanine  4.960      
L-Proline  34.500      
L-Serine  10.500      
L-Threonine  11.900      
L-Tryptophan  2.040      
L-Tyrosine disodium salt  7.810      
L-Valine  11.700      


Directions:
 
  1. Add 20ml of 200mM L-glutamine (TCL012) or HiGlutaXL™ supplement (TCL030) for 1 litre of medium.

Recommendations for use with Losera™ Media:
 
  1. LoSera™ media have been optimized at 2.5% serum concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. LoSera™ media are provided as 1X solutions and need to be supplemented with 4mM Glutamine and required amount of reduced serum.
  3. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotics in proportion to the amount of serum reduced.


Material required but not provided:
 
  • L-Glutamine Solution 200mM (TCL012)
  • HiGlutaXL™ Supplement (TCL030)
  • Fetal Bovine Serum (RM1112/RM10432)  


Quality Control:
 
  • Appearance: Orangish colored, clear solution
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 270.00 -310.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml
 
Storage and Shelf Life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
     
 

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