LoSeraTM Nutrient Mixture F-10 Ham w/ Sodium bicarbonate w/o L-Glutamine (Liquid requiring reduced serum supplementation)

Product#: HM-RSL009
$68.08
HM-RSL009
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LoSera™ Nutrient Mixture F-10 Ham
With Sodium bicarbonate
Without L-Glutamine
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-RSL009

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

RSL009 is LoSera™ Nutrient Mixture F-10 Ham medium with Sodium bicarbonate. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  44.100   Choline chloride  0.024
Copper sulphate pentahydrate  0.0025   D-Biotin  0.698
Ferric sulphate heptahydrate  0.834   D-Ca-Pantothenate  0.715
Magnesium sulphate anhydrous  74.730   Folic acid  1.320
Potassium chloride  285.000   Niacinamide  0.615
Potassium dihydrogen phosphate  83.000   Pyridoxine hydrochloride  0.206
Sodium bicarbonate  1200.000   Riboflavin  0.376
Sodium chloride  7400.000   Thiamine hydrochloride  1.000
Sodium phosphate dibasic anhydrous  153.700   Vitamin B12  1.360
Zinc sulphate heptahydrate  0.0288   i-Inositol  0.541
AMINO ACIDS     OTHERS  
Glycine 7.510   D-Glucose  1100.000
L-Alanine  8.910   Fetal Bovine Serum  Proprietary
L-Arginine hydrochloride  211.000   Hypoxanthine sodium salt  4.080
L-Asparagine anhydrous  15.010   Lipoic acid  0.210
L-Aspartic acid  13.300   Penicillin  Proprietary
L-Cystine dihydrochloride  35.130   Phenol red sodium salt  1.300
L-Glutamic acid  14.700   Streptomycin  Proprietary
L-Glutamine  146.000   Sodium pyruvate  110.000
L-Histidine hydrochloride monohydrate  21.000   Thymidine  0.730
L-Isoleucine  2.600      
L-Leucine  13.100      
L-Lysine hydrochloride  29.300      
L-Methionine  4.480      
L-Phenylalanine  4.960      
L-Proline  11.500      
L-Serine  10.500      
L-Threonine  3.570      
L-Tryptophan  0.600      
L-Tyrosine disodium salt  2.610      
L-Valine  3.500      


Directions:
 
  1. Add 20ml of 200mM L-glutamine (TCL012) or HiGlutaXL™ supplement (TCL030) for 1 litre of medium.

Recommendations for use with Losera™ Media:
 
  1. LoSera™ media have been optimized at 2.5% serum concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. LoSera™ media are provided as 1X solutions and need to be supplemented with 4mM Glutamine and required amount of reduced serum.
  3. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotics in proportion to the amount of serum reduced.


Material required but not provided:
 
  • L-Glutamine Solution 200mM (TCL012)
  • HiGlutaXL™ Supplement (TCL030)
  • Fetal Bovine Serum (RM1112/RM10432)


Quality Control:
 
  • Appearance: Orangish colored, clear solution
  • pH: 7.00 - 7.60
  • Osmolality in mOsm/Kg H2O: 285.00 - 325.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
 
Storage and Shelf Life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
     
 

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