LoSeraTM Minimum Essential Medium Eagle w/ Earle’s salts, NEAA and Sodium bicarbonate w/o L-Glutamine (Liquid requiring reduced serum supplementation)

Product#: HM-RSL008
$52.30
HM-RSL008
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LoSera™ Minimum Essential Medium Eagle
With Earle’s salts, Sodium bicarbonate and NEAA
Without L-Glutamine
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-AL008

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

LoSera™ Minimum Essential Medium Eagle is medium with Earle's salts, NEAA and Sodium bicarbonate. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium nitrate dihydrate 265.000   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Biotin  0.100
Potassium chloride  400.000   D-Ca-Pantothenate  1.000
Sodium bicarbonate  2200.000   Folic acid  1.000
Sodium chloride 6800.000   Nicotinamide  1.000
Sodium dihydrogen phosphate anhydrous  122.000   Pyridoxal hydrochloride  1.000
AMINO ACIDS     Riboflavin  0.100
Glycine 7.500   Thiamine hydrochloride  1.000
L-Alanine  8.900   i-Inositol  2.000
L-Arginine hydrochloride  126.000   OTHERS  
L-Asparagine monohydrate 15.000   D-Glucose  1000.000
L-Aspartic acid  13.300   Growth Supplement mix  Proprietary
L-Cysteine dihydrochloride  31.300   Phenol red sodium salt  11.000
L-Cystine dihydrochloride  31.300      
L-Glutamic acid  14.700      
L-Histidine hydrochloride monohydrate 42.000      
L-Isoleucine  52.000      
L-Leucine  52.000      
L-Lysine hydrochloride  72.500      
L-Methionine  15.000      
L-Phenylalanine  32.000      
L-Proline  40.000      
L-Serine  25.000      
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      


Directions:
 
  1. Add 20ml of 200mM L-glutamine (TCL012) or HiGlutaXL™ supplement (TCL030) for 1 litre of medium.

Recommendations for use with LoSera™ Media:
 
  1. LoSera™ media have been optimized at 2.5% serum concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. LoSera™ media are provided as 1X solutions and need to be supplemented with 4mM Glutamine and required amount of reduced serum. 3. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotics in proportion to the amount of serum reduced.


Material required but not provided:
 
  • L-Glutamine solution 200mM (TCL012)
  • HiGlutaXL™ Supplement (TCL030)
  • Fetal Bovine Serum (RM1112/RM10432)


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
 

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