LoSeraTM Iscove’s Modified Dulbecco’s Medium w/ HEPES buffer and Sodium bicarbonate w/o L-Glutamine (Liquid requiring reduced serum supplementation)

Product#: HM-RSL007
$68.53
HM-RSL007
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LoSera™ Iscove's Modified Dulbecco's Medium
With 25mM HEPES Buffer and Sodium bicarbonate
Without L-Glutamine
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-RSL007

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

RSL007 is LoSera™ Iscove's Modified Dulbecco's Medium with sodium bicarbonate and HEPES buffer. It does not contain L-glutamine. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  219.000   Choline chloride  4.000
Magnesium sulphate anhydrous  97.720   D-Biotin  0.013
Potassium chloride  330.000   D-Ca-Pantothenate  4.000
Potassium nitrate  0.076   Folic acid  4.000
Sodium bicarbonate  3204.000   Nicotinamide  4.000
Sodium chloride  4505.000   Pyridoxal hydrochloride  4.000
Sodium dihydrogen phosphate anhydrous  109.000   Riboflavin  0.400
Sodium selenite  0.0173   Thiamine hydrochloride  4.000
AMINO ACIDS     Vitamin B12  0.013
Glycine 30.000   i-Inositol  7.200
L-Alanine  25.000   OTHERS  
L-Arginine hydrochloride  84.000   D-Glucose  4500.000
L-Asparagine  25.000   Growth Supplement mix  Proprietary
L-Aspartic acid  30.000   HEPES Buffer  5958.000
L-Cysteine dihydrochloride  91.240   Phenol red (Sodium Salt)  15.000
L-Glutamic acid  75.000   Sodium pyruvate  110.000
L-Histidine hydrochloride monohydrate  42.000      
L-Isoleucine  104.800      
L-Leucine  104.800      
L-Lysine hydrochloride  146.200      
L-Methionine  30.000      
L-Phenylalanine  66.000      
L-Proline  40.000      
L-Serine  42.000      
L-Threonine  95.200      
L-Tryptophan  16.000      
L-Tyrosine disodium salt  104.200      
L-Valine  93.600      


Directions:
 
  1. Add 20ml of 200mM L-glutamine (TCL012) or HiGlutaXL™ supplement (TCL030) for 1 litre of medium.

Recommendations for use with LoSera™ Media:
 
  1. LoSera™ media have been optimized at 2.5% serum Recommendations for use with LoSera™ Media: concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. LoSera™ media are provided as 1X solutions and need to be supplemented with 4mM Glutamine and required amount of reduced serum. 3. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotics in proportion to the amount of serum reduced.


Material required but not provided:
 
  • L-Glutamine solution 200mM (TCL012)
  • HiGlutaXL™ Supplement (TCL030)
  • Fetal Bovine Serum (RM1112/RM10432)


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
 

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