LoSeraTM Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (1:1) w/ Trace elements and Sodium bicarbonate w/o L-Glutamine and HEPES buffer (Liquid)

Product#: HM-RSL005
$60.29
HM-RSL005
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LoSera™ Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture)
With Trace elements and Sodium bicarbonate
Without L-Glutamine and HEPES buffer
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-RSL005

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

RSL005 is LoSera™ DMEM/Nutrient Mixture F-12 Ham with trace elements and sodium bicarbonate. It does not contain L-glutamine and HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Ammonium metavanadate  0.00058   Choline chloride  8.980
Ammonium molybdate tetrahydrate  0.00618   D-Biotin  0.0035
Calcium chloride dihydrate  154.500   D-Ca-Pantothenate  2.240
Copper sulphate pentahydrate  0.0013   Folic acid  2.240
Disodium hydrogen phosphate  71.020   Niacinamide  2.020
Ferric nitrate nonahydrate  0.050   Pyridoxal hydrochloride  2.000
Ferrous sulphate heptahydrate  0.417   Pyridoxine hydrochloride  0.031
Magnesium chloride hexahydrate  61.200   Riboflavin  0.219
Magnesium sulphate anhydrous  48.840   Thiamine hydrochloride  2.170
Manganese sulphate  0.000151   Vitamin B12  0.680
Nickel chloride  0.00012   myo-Inositol  12.600
Sodium bicarbonate  1200.000   OTHERS  
Potassium chloride  311.800   D-Glucose  3151.000
Sodium chloride  6996.000   DL-Thioctic acid  0.105
Sodium dihydrogen phosphate  54.300   Growth Supplement mix  Proprietary
Sodium metasillicate nonahydrate  0.0142   HEPES buffer  3574.500
Sodium selenite  0.00519   Hypoxanthine  2.400
Stannous chloride dihydrate  0.00011   Linoleic acid  0.042
Zinc sulphate heptahydrate  0.432   Phenol red sodium salt  8.630
AMINO ACIDS     Putrescine hydrochloride  0.081
Glycine 18.750   Sodium pyruvate  110.000
L-Alanine  4.450   Thymidine 0.365
L-Arginine hydrochloride  147.500      
L-Asparagine monohydrate  7.500      
L-Aspartic acid  6.650      
L-Cysteine dihydrochloride  17.560      
L-Cystine hydrochloride monohydrate  31.290      
L-Glutamic acid  7.350      
L-Alanyl-L-glutamine  543.000      
L-Histidine hydrochloride monohydrate  31.480      
L-Isoleucine  54.470      
L-Leucine  59.050      
L-Lysine hydrochloride  91.250      
L-Methionine  17.240      
L-Phenylalanine  35.480      
L-Proline  17.250      
L-Serine  26.250      
L-Threonine  53.450      
L-Tryptophan  9.020      
L-Tyrosine disodium salt  48.100      
L-Valine  52.850      


Directions:
 
  1. Add 20ml of 200mM L-glutamine (TCL012) or HiGlutaXL™ supplement (TCL030) for 1 litre of medium.

Recommendations for use with LoSera™ Media:
 
  1. LoSera™ media have been optimized at 2.5% serum concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. LoSera™ media are provided as 1X solutions and need to be supplemented with 4mM L-glutamine and required amount of reduced serum. 3. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotics in proportion to the amount of serum reduced.


Material required but not provided:
 
  • L-Glutamine solution 200mM (TCL012)
  • HiGlutaXL™ supplement (TCL030)
  • Fetal Bovine Serum (RM1112/RM10432)


Quality Control:
 
  • Appearance: Red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 310.00 -350.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
 

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