LoSeraTM Dulbecco’s Modified Eagle Medium, High Glucose w/ L-Alanyl-L-Glutamine, Sodium pyruvate and Sodium bicarbonate (Liquid requiring reduced serum supplementation)

Product#: HM-RSL003G
$54.94
HM-RSL003G
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LoSera™ Dulbecco's Modified Eagle Medium, High Glucose
With L-Alanyl-L-Glutamine, Sodium Pyruvate and Sodium bicarbonate
1X Liquid Cell Culture Medium requiring reduced serum supplementation

Product Code: HM-RSL003G

Product Description:


LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow most of the common cell lines. The percentage of serum reduction may vary with type of cell line used. For nonfastidious cell lines serum can be reduced from 10 % to as low as 1%. For fastidious cell lines serum usage can be reduced from 10 % to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

RSL003G is LoSera™ Dulbecco's Modified Eagle Medium with sodium pyruvate, sodium bicarbonate and L-Alanyl-L-Glutamine. L-alanyl-L-glutamine is the stabilized dipeptide form of L-glutamine. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  265.000   Choline chloride  4.000
Ferric nitrate nonahydrate  0.100   D-Ca-Pantothenate  4.000
Magnesium sulphate anhydrous  97.720   Folic acid  4.000
Potassium chloride  400.000   Nicotinamide 4.000
Sodium bicarbonate  3700.000   Pyridoxal hydrochloride  4.000
Sodium chloride  6400.000   Riboflavin 0.400
Sodium dihydrogen phosphate anhydrous  109.000   Thiamine hydrochloride  4.000
AMINO ACIDS     i-Inositol  7.200
Glycine 30.000   OTHERS  
L-Arginine hydrochloride  84.000   D-Glucose  4500.000
L-Cysteine dihydrochloride  62.570   Growth Supplement mix  Proprietary
L-Histidine hydrochloride  42.000   Phenol red sodium salt  15.900
L-Hydroxyproline  20.000   Sodium pyruvate  110.000
L-Isoleucine  105.000      
L-Leucine  105.000      
L-Lysine hydrochloride  146.000      
L-Methionine  30.000      
L-Phenylalanine  66.000      
L-Serine  42.000      
L-Threonine  95.000      
L-Tryptophan  16.000      
L-Tyrosine disodium salt  103.790      
L-Valine  94.000      


Directions:

Recommendations for use with LoSera™ Media:
  1. LoSera™ media have been optimized at 2.5% serum concentration for a broad range of cell culture applications. Recommended concentrations of serum using LoSera™ media ranges from 1-5%. However the concentration of serum used may need to be adjusted for specific cell types or applications to achieve optimal results. Titration of FBS concentration is recommended to determine maximum serum reduction.
  2. In case of antibiotics being used to control contamination, it is recommended to reduce the amount of antibiotic in proportion to the amount of serum reduced.


Material required but not provided:
 
  • Fetal Bovine Serum (RM1112/RM10432)


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 320.00 -360.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 12 months.
  • Use before expiry date given on the product label.
 

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