HiGlutaXLTM RPMI-1640 w/ L-Alanyl-L-Glutamine and Sodium bicarbonate (Liquid)

Product#: HM-AL028G
$32.67
HM-AL028G
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HiGlutaXL™ RPMI-1640
w/ L-Alanyl-L-Glutamine and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL028G

Product Description:


HiGlutaXLTM medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXLTM medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 40 C for longer periods.

AL028G is HiGlutaXLTM RPMI 1640 with L-alanyl-Lglutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients   mg/L
Calcium nitrate tetrahydrate    100.00
Magnesium sulphate anhydrous    48.84
Potassium chloride    400.00
Sodium chloride    6,000.00
Sodium phosphate, dibasic anhydrous    800.00
D-Glucose    2,000.00
Glutathione reduced    1.00
Phenol red .Na salt    5.30
L-Arginine Hydrochloride    241.00
L-Asparagine    50.00
L-Aspartic acid    20.00
L-Cystine dihydrochloride    65.20
L-Glutamic acid    20.00
L-Alanyl-L-Glutamine    446.00
Glycine   10.00
L-Histidine Hydrochloride monohydrate   20.96
L-Hydroxyproline    20.00
L-Isoleucine    50.00
L-Leucine    50.00
L-Lysine hydrochloride    40.00
L-Methionine    15.00
L-Phenylalanine    15.00
L-Proline    20.00
L-Serine    30.00
L-Threonine    20.00
L-Tryptophan    5.00
L-Tyrosine (Na Salt)    28.83
L-Valine    20.00
D-Biotin    0.20
D-Ca-Pantothenate    0.25
Choline chloride    3.00
Folic acid    1.00
i-Inositol    35.00
Niacinamide    1.00
p-Amino benzoic acid (PABA)    1.00
Thiamine hydrochloride    1.00
Vitamin B12    0.005
Pyridoxine hydrochloride    1.00
Riboflavin    0.20
Sodium bicarbonate    2000.00


Quality Control:
 
  • Appearance:Orangish red colored, clear solution.
  • pH at 25oC:7.3± 0.3
  • Osmolality in mOsm/Kg H2O: 290- 330
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: Growth promotion : The growth promotion capacity of the medium is assessed by supplementing the medium with 10% fetal bovine serum and checking the growth using an appropriate cell line and comparing it with a control medium. Growth promotion studies are carried out through two or more subcultures. The medium is analyzed for its growth supporting property qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts. The medium is also tested for its good anchoraging capacity and buffering action. Toxicity on cell lines: Negative
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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