HiGlutaXLTM RPMI-1640 With L-Alanyl-L-Glutamine, HEPES buffer and Sodium bicarbonate (Liquid)

Product#: HM-AL060G
$43.06
HM-AL060G
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HiGlutaXL™ RPMI-1640
With L-Alanyl-L-Glutamine, HEPES buffer and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL060G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-Glutamine, L-Alanyl-L-Glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-Alanyl-LGlutamine incorporates L-Alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-Glutamine and L-Alanine for use by the cell. The gradual release of LGlutamine obviates the need to supplement L-Glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL060G is HiGlutaXL™ RPMI-1640 medium supplemented with 25mM HEPES buffer, L-alanylL-glutamine and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37° C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium nitrate tetrahydrate  100.000   Choline chloride  3.000
Disodium hydrogen phosphate anhydrous  800.000   D-Biotin  0.200
Magnesium sulphate anhydrous  48.840   D-Ca-Pantothenate  0.250
Potassium chloride  400.000   Folic acid  1.000
Sodium bicarbonate  2000.000   Niacinamide  1.000
Sodium chloride  6000.000   Pyridoxine hydrochloride  1.000
AMINO ACIDS     Riboflavin  0.200
Glycine 10.000   Thiamine hydrochloride  1.000
L-Alanyl-L-Glutamine  446.000   Vitamin B12  0.005
L-Arginine hydrochloride  241.000   i-Inositol  35.000
L-Asparagine anhydrous  50.000   p-Amino benzoic acid (PABA)  1.000
L-Aspartic acid  20.000   OTHERS  
L-Cysteine dihydrochloride  65.200   D-Glucose  2000.000
L-Glutamic acid  20.000   Glutathione reduced  1.000
L-Histidine hydrochloride  20.960   HEPES Buffer  5958.000
L-Hydroxyproline  20.000   Phenol red sodium salt  5.300
L-Isoleucine  50.000      
L-Leucine  50.000      
L-Lysine hydrochloride  40.000      
L-Methionine  15.000      
L-Phenylalanine  15.000      
L-Proline  20.000      
L-Serine  30.000      
L-Threonine  20.000      
L-Tryptophan  5.000      
L-Tyrosine disodium salt  28.830      
L-Valine  20.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 320.00 -360.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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