HiGlutaXLTM Nutrient Mixture F-12 Ham w/ L-Alanyl-L-Glutamine and Sodium bicarbonate (Liquid)

Product#: HM-AL025G
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HiGlutaXL™ Nutrient Mixture F-12 Ham
With L-Alanyl-L-Glutamine and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL025G

Product Description:

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL025G is HiGlutaXL™ Nutrient Mixture F-12 Ham with L-alanyl-L-glutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Ingredients mg/L   Ingredients mg/L
Calcium chloride dihydrate  44.100   Biotin  0.0073
Copper sulphate pentahydrate  0.0025   Choline chloride  13.960
Ferric sulphate heptahydrate  0.834   D-Ca-Pantothenate  0.480
Magnesiumchloride anhydrous 57.650   Folic acid  1.320
Potassium chloride  223.600   Nicotinamide  0.615
Potassium chloride  223.600   Pyridoxine hydrochloride  0.062
Sodium bicarbonate  1176.000   Riboflavin  0.038
Sodium chloride  7599.000   Thiamine hydrochloride  0.340
Sodium phosphate dibasic anhydrous  142.040   Vitamin B12  1.360
Zinc sulphate tetrahydrate 0.863   i-Inositol  18.000
Glycine 7.500   D-Glucose  1801.600
L-Alanine  8.910   Hypoxanthine sodium salt  4.770
L-Alanyl-L-Glutamine  217.000   Linoleic acid  0.084
L-Arginine hydrochloride  210.700   Lipoic acid  0.210
L-Asparagine anhydrous  15.010   Phenol red sodium salt  1.240
L-Aspartic acid  13.300   Putrescine dihydrochloride  0.161
L-Cysteine dihydrochloride  17.560   Sodium pyruvate  110.100
L-Cystine hydrochloride  35.120   Thymidine  0.730
L-Glutamic acid  14.700      
L-Histidine hydrochloride monohydrate  20.960      
L-Isoleucine  3.940      
L-Leucine  13.100      
L-Lysine hydrochloride  36.500      
L-Methionine  4.480      
L-Phenylalanine  4.960      
L-Proline  34.500      
L-Serine  10.500      
L-Threonine  11.900      
L-Tryptophan  2.040      
L-Tyrosine disodium salt  7.810      
L-Valine  11.700      

Quality Control:
  • Appearance:Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 300.00 -340.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml

Storage and shelf life:
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.

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