HiGlutaXLTM Nutrient Mixture F-10 Ham w/ L-Alanyl-L-Glutamine and Sodium bicarbonate (Liquid)

Product#: HM-AL024G
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HiGlutaXL™ Nutrient Mixture F-10 Ham

With L-Alanyl-L-Glutamine and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL024G

Product Description:

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL024G is HiGlutaXL™ Nutrient Mixture F-10 Ham with sodium bicarbonate and L-alanyl-L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Ingredients mg/L   Ingredients mg/L
Calcium chloride dihydrate  44.100   Biotin  0.024
Copper sulphate pentahydrate  0.0025   Choline chloride  0.698
Ferric sulphate heptahydrate  0.834   D-Ca-Pantothenate  0.715
Magnesium sulphate anhydrous  74.730   Folic acid  1.320
Potassium chloride  285.000   Nicotinamide  0.615
Potassium dihydrogen phosphate  83.000   Pyridoxine hydrochloride  0.206
Sodium bicarbonate  1200.000   Riboflavin  0.376
Sodium chloride  7400.000   Thiamine hydrochloride  1.000
Sodium phosphate dibasic anhydrous  153.700   Vitamin B12  1.360
Zinc sulphate heptahydrate  0.0288   i-Inositol  0.541
Glycine 7.510   D-Glucose  1100.000
L-Alanine  8.910   Hypoxanthine sodium salt  4.080
L-Alanyl-L-Glutamine  217.000   Lipoic acid  0.210
L-Arginine hydrochloride  211.000   Phenol red sodium salt  1.300
L-Asparagine anhydrous  15.010   Sodium pyruvate  110.000
L-Aspartic acid  13.300   Thymidine  0.730
L-Cysteine dihydrochloride  17.560      
L-Cystine hydrochloride monohydrate  35.130      
L-Glutamic acid  14.700      
L-Histidine hydrochloride monohydrate  21.000      
L-Isoleucine  2.600      
L-Leucine  13.100      
L-Lysine hydrochloride  29.300      
L-Methionine  4.480      
L-Phenylalanine  4.960      
L-Proline  11.500      
L-Serine  10.500      
L-Threonine  3.570      
L-Tryptophan  0.600      
L-Tyrosine disodium salt  2.610      
L-Valine  3.500      

Quality Control:
  • Appearance:Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 285.00 -325.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml

Storage and shelf life:
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.

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