HiGlutaXLTM Minimum Essential Medium Eagle w/ L-Alanyl-L-Glutamine, Hank’s salts and Sodium bicarbonate (Liquid)

Product#: HM-AL056G
$32.67
HM-AL056G
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HiGlutaXL™ Minimum Essential Medium Eagle
With Hank's salts, L-Alanyl-L-Glutamine and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL056G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL056G is HiGlutaXL™ Minimum Essential Medium with L-alanyl-L-glutamine, Hank's balanced salts and sodium bicarbonate. Hank's salt mixture is designed to equilibrate with air, hence does not require CO2 air mixture. Cells can therefore be grown in AL056G in less CO2 or CO2 free environment. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  185.410   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Ca-Pantothenate  1.000
Potassium chloride  400.000   Folic acid  1.000
Potassium phosphate monobasic 60.000   Nicotinamide 1.000
Sodium bicarbonate  350.000   Pyridoxal hydrochloride  1.000
Sodium chloride  8000.000   Riboflavin 0.100
Sodium phosphate dibasic anhydrous  47.800   Thiamine hydrochloride 1.000
AMINO ACIDS     i-Inositol  2.000
L-Alanyl-L-Glutamine  434.000   OTHERS  
L-Arginine hydrochloride  126.000   D-Galactose 1000.000
L-Cystine dihydrochloride  31.300   Phenol red sodium salt  11.000
L-Histidine hydrochloride  42.000      
L-Isoleucine  52.000      
L-Leucine  52.000      
L-Lysine hydrochloride  72.500      
L-Methionine  15.000      
L-Phenylalanine  32.000      
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      


Quality Control:
 
  • Appearance: Red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 290.00 -330.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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