HiGlutaXLTM Minimum Essential Medium Eagle With Earle's salts, L-Alanyl-L-Glutamine, NEAA and Sodium bicarbonate (Liquid)

Product#: HM-AL047G
$32.67
HM-AL047G
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HiGlutaXL™ Minimum Essential Medium Eagle
With Earle's salts, L-Alanyl-L-Glutamine, NEAA and Sodium bicarbonate
1X Liquid cell Culture Medium

Product Code: HM-AL047G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need of addition of L-glutamine at the time of use and allows liquid media to be stored at 4°C for longer periods.

AL047G is HiGlutaXL™ Minimum Essential Medium Eagle with Earle's salts, L-alanyl-L-glutamine, sodium bicarbonate and non-essential amino acids. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  265.000   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Ca-Pantothenate  1.000
Potassium chloride  400.000   Folic acid  1.000
Sodium bicarbonate  2200.000   Nicotinamide  1.000
Sodium chloride  6800.000   Pyridoxal hydrochloride  1.000
Sodium dihydrogen phosphate anhydrous  122.000   Riboflavin  0.100
AMINO ACIDS     i-Inositol  2.000
Glycine 7.500   OTHERS  
L-Alanine  8.900   D-Glucose  1000.000
L-Alanyl-L-Glutamine  434.000   Phenol red sodium salt  11.000
L-Arginine hydrochloride  126.000      
L-Asparagine monohydrate  15.000      
L-Aspartic acid  13.300      
L-Cysteine dihydrochloride  31.300      
L-Glutamic acid  14.700      
L-Histidine hydrochloride monohydrate  42.000      
L-Isoleucine  52.000      
L-Leucine  52.000      
L-Lysine hydrochloride  72.500      
L-Methionine  15.000      
L-Phenylalanine  32.000      
L-Proline  11.500      
L-Serine  10.500      
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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