HiGlutaXLTM Minimum Essential Medium Eagle (MEM) w/ L-Alanyl-L-Glutamine, Earle’s salt and Sodium bicarbonate (Liquid)

Product#: HM-AL020G
$47.65
HM-AL020G
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HiGlutaXL™ Minimum Essential Medium Eagle (MEM)
With L-Alanyl-L-Glutamine, Earle's salt and Sodium bicarbonate
Without NEAA 1X Liquid Cell Culture Medium

Product Code: HM-AL080G

Product Description:


HiGlutaXL™ media contain the stabilized dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ media offer several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL020G is HiGlutaXL™ Minimum Essential Medium Eagle with L-alanyl-L-glutamine, Earle's salts and sodium bicarbonate. It does not contain non-essential amino acids. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium nitrate dihydrate 265.000   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Ca-Pantothenate  1.000
Potassium chloride  400.000   Folic acid  1.000
Sodium bicarbonate  2200.000   Nicotinamide 1.000
Sodium chloride 6800.000   Pyridoxal hydrochloride  1.000
Sodium dihydrogen phosphate anhydrous  122.000   Riboflavin  0.100
AMINO ACIDS     Thiamine hydrochloride  1.000
L-Arginine hydrochloride  126.000   i-Inositol  2.000
L-Cystine dihydrochloride  31.300   OTHERS  
L-Histidine hydrochloride monohydrate  42.000   D-Glucose  1000.000
L-Isoleucine  52.000   L-Alanyl-L-Glutamine  434.000
L-Leucine  52.000   Phenol red sodium salt  11.000
L-Lysine hydrochloride  72.500      
L-Methionine  15.000      
L-Phenylalanine  32.000      
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 290.00 -330.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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