HiGlutaXLTM Minimum Essential Medium (Alpha Modification) w/ L-Alanyl-L-Glutamine and Sodium bicarbonate w/o Deoxyribonucleosides and Ribonucleosides (Liquid)

Product#: HM-AL081G
$39.14
HM-AL081G
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HiGlutaXL™ Minimum Essential Medium Eagle (Alpha modification)
With L-Alanyl-L-Glutamine, Sodium bicarbonate
Without Deoxyribonucleosides and Ribonucleosides
1X Liquid Cell Culture Medium

Product Code: HM-AL081G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL081G is alpha modification of HiGlutaXL™ Minimum Essential Medium Eagle with L-alanylL-glutamine, sodium bicarbonate and non-essential amino acid. It is devoid of deoxyribonucleosides and ribonucleosides. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium nitrate dihydrate 265.000   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Biotin  0.100
Potassium chloride  400.000   D-Ca-Pantothenate  1.000
Sodium bicarbonate  2200.000   Folic acid  1.000
Sodium chloride 6800.000   L-Ascorbic acid  50.000
Sodium dihydrogen phosphate anhydrous  122.000   Nicotinamide  1.000
AMINO ACIDS     Pyridoxal hydrochloride  0.200
Glycine 50.000   Riboflavin 0.100
L-Alanine  25.000   Thiamine hydrochloride  1.000
L-Alanyl-L-Glutamine  434.000   Vitamin B12  1.360
L-Arginine hydrochloride  126.000   i-Inositol  2.000
L-Asparagine monohydrate 44.000   OTHERS  
L-Aspartic acid  30.000   D-Glucose  1000.000
L-Cysteine dihydrochloride  100.000   Lipoic acid  0.200
L-Cystine dihydrochloride  31.300   Phenol red sodium salt  11.000
L-Glutamic acid  75.000   Sodium pyruvate  110.000
L-Histidine hydrochloride monohydrate 42.000      
L-Isoleucine  52.000      
L-Leucine  52.000      
L-Lysine hydrochloride  72.500      
L-Methionine  15.000      
L-Phenylalanine  32.000      
L-Proline  40.000      
L-Serine  25.000      
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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