HiGlutaXLTM Minimum Essential Medium (Alpha Modification) w/ L-Alanyl-L-Glutamine, Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate (Liquid)

Product#: HM-AL080G
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HiGlutaXL™ Minimum Essential Medium Eagle (Alpha modification)
With L-Alanyl-L-Glutamine, Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL080G

Product Description:

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXLTM medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL080G is alpha modification of HiGlutaXL™ Minimum Essential Medium Eagle with Lalanyl-L-glutamine, NEAA, deoxyribonucleosides, ribonucleosides and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Ingredients mg/L   Ingredients mg/L
Calcium nitrate dihydrate 265.000   Choline chloride  1.000
Magnesium sulphate anhydrous  97.720   D-Biotin  0.100
Potassium chloride  400.000   D-Ca-Pantothenate  1.000
Sodium bicarbonate  2200.000   Folic acid  1.000
Sodium chloride 6800.000   L-Ascorbic acid  50.000
Sodium dihydrogen phosphate anhydrous  122.000   Nicotinamide  1.000
AMINO ACIDS     Pyridoxal hydrochloride  0.200
Glycine 50.000   Riboflavin 0.100
L-Alanine  25.000   Thiamine hydrochloride  1.000
L-Alanyl-L-Glutamine  434.000   Vitamin B12  1.360
L-Arginine hydrochloride  126.000   i-Inositol  2.000
L-Asparagine monohydrate 44.000   OTHERS  
L-Aspartic acid  30.000   2' Deoxyadenosine  10.000
L-Cysteine dihydrochloride  100.000   2' Deoxycytidine hydrochloride  11.000
L-Cystine dihydrochloride  31.300   2' Deoxyguanosine  10.000
L-Glutamic acid  75.000   Adenosine  10.000
L-Histidine hydrochloride monohydrate 42.000   Cytidine  10.000
L-Isoleucine  52.000   D-Glucose  1000.000
L-Leucine  52.000   Guanosine  10.000
L-Lysine hydrochloride  72.500   Lipoic acid  0.200
L-Methionine  15.000   Phenol red sodium salt  11.000
L-Phenylalanine  32.000   Sodium pyruvate  110.000
L-Proline  40.000   Thymidine  10.000
L-Serine  25.000   Uridine  10.000
L-Threonine  48.000      
L-Tryptophan  10.000      
L-Tyrosine disodium salt  51.900      
L-Valine  46.000      

Quality Control:
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml

Storage and shelf life:
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.

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