HiGlutaXLTM Medium 199 w/ Earle's salts, L-Alanyl-L-Glutamine and Sodium bicarbonate (Liquid)

Product#: HM-AL014G
Ships in 1-2 weeks


HiGlutaXL™ Medium 199
With L-Alanyl-L-Glutamine, Earle's Salts and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL014G

Product Description:

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both Lglutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement Lglutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL014G is HiGlutaXL™ Medium 199 with L-alanylLglutamine, Earle's salts and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Ingredients mg/L   Ingredients mg/L
Calcium chloride dihydrate  265.000   Ascorbic acid  0.050
Ferric nitrate nonahydrate  0.720   Calciferol  0.100
Magnesium sulphate anhydrous  97.720   Choline chloride  0.500
Potassium chloride 400.000   D-Biotin  0.010
Sodium acetate anhydrous 50.000   D-Ca-Pantothenate  0.010
Sodium bicarbonate 2200.000   DL-Tocopherol phosphate disodium salt  0.010
Sodium chloride 6800.000   Folic acid  0.010
Sodium phosphate monobasic 122.000   Menadione  0.010
AMINO ACIDS     Nicotinamide  0.025
Glycine 50.000   Nicotinic acid  0.025
L-Alanine  25.000   Pyridoxal hydrochloride  0.025
L-Alanyl-L-Glutamine  148.782   Pyridoxine hydrochloride  0.025
L-Arginine hydrochloride  70.000   Retinol acetate  0.140
L-Aspartic acid  30.000   Riboflavin  0.010
L-Cysteine dihydrochloride  26.000   Thiamine hydrochloride  0.010
L-Glutamic acid  67.000   i-Inositol  0.050
L-Histidine hydrochloride monohydrate  22.000   p-Amino benzoic acid (PABA)  0.050
L-Hydroxyproline  10.000   OTHERS  
L-Isoleucine  20.000   Adenine sulphate  10.000
L-Leucine  60.000   Adenosine monophosphate  0.200
L-Lysine hydrochloride  70.000   Adenosine triphosphate  1.000
L-Methionine  15.000   Cholesterol  0.200
L-Phenylalanine  25.000   Deoxyribose  0.5000
L-Proline  40.000   Glucose  1000.000
L-Serine  25.000   Glutathione reduced  0.050
L-Threonine  30.000   Guanine hydrochloride  0.300
L-Tryptophan  10.000   Hypoxanthine  0.354
L-Tyrosine disodium salt  57.660   Phenol red sodium salt  15.000
L-Valine  25.000   Polysorbate 80 4.900
      Ribose 0.500
      Thymine  0.300
      Uracil  0.300
      Xanthine  0.344

Quality Control:
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 1EU/ml

Storage and shelf life:
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.

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