HiGlutaXLTM Leibovitz’s L-15 Medium w/ L-Alanyl-L-Glutamine (Liquid)

Product#: HM-AL011G
$47.65
HM-AL011G
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HiGlutaXL™ Leibovitz's L-15 Medium
With L-Alanyl-L-Glutamine
1X Liquid Cell Culture Medium

Product Code: HM-AL011G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4ºC for longer periods.

AL011G is HiGlutaXL™ Leibovitz's Medium with L-alanyl-L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  185.000   Choline chloride  1.000
Magnesium chloride hexahydrate  200.000   D-Ca-Pantothenate  1.000
Magnesium sulphate anhydrous  97.720   Folic acid  1.000
Potassium chloride  400.000   Nicotinamide 1.000
Potassium phosphate monobasic  60.000   Pyridoxal hydrochloride  1.000
Sodium chloride  8000.000   Riboflavin-5-phosphate sodium salt  0.100
Sodium dihydrogen phosphate anhydrous  109.120   Thiamine monophosphate 1.000
AMINO ACIDS     i-Inositol  2.000
D-Alpha alanine  450.000   OTHERS  
Glycine 200.000   D-Galactose 900.000
L-Alanyl-L-Glutamine  446.000   Phenol red sodium salt  11.000
L-Arginine (free base) 500.000   Sodium pyruvate  550.000
L-Asparagine  250.000      
L-Cysteine (free base) 120.000      
L-Histidine (free base)  250.000      
L-Isoleucine  250.000      
L-Leucine  125.000      
L-Lysine   146.000      
L-Lysine hydrochloride  94.000      
L-Methionine  75.000      
L-Phenylalanine  125.000      
L-Serine  200.000      
L-Threonine  300.000      
L-Tryptophan  20.000      
L-Tyrosine disodium salt  276.160      
L-Valine  100.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.30 -7.90
  • Osmolality in mOsm/Kg H2O: 300.00 -340.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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