HiGlutaXLTM Iscove’s Modified Dulbecco’s Medium w/ L-Alanyl-L-Glutamine, HEPES buffer and Sodium bicarbonate (Liquid)

Product#: HM-AL070G
$39.14
HM-AL070G
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HiGlutaXL™ Iscove's Modified Dulbecco's Medium
With L-Alanyl-L-Glutamine, HEPES Buffer and Sodium bicarbonate
1X Liquid Cell Culture Medium

Product Code: HM-AL070G

Product Description:


HiGlutaXL™ medium contains the stabilized dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL070G is HiGlutaXL™ Iscove's Modified Dulbecco's Medium with L-alanyl-L-glutamine, 25mM HEPES buffer and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  219.000   Choline chloride  4.000
Magnesium sulphate anhydrous  97.720   D-Biotin  0.013
Potassium chloride  330.000   D-Ca-Pantothenate  4.000
Potassium nitrate  0.076   Folic acid  4.000
Sodium bicarbonate  3204.000   Nicotinamide  4.000
Sodium chloride  4505.000   Pyridoxal hydrochloride  4.000
Sodium dihydrogen phosphate anhydrous  109.000   Riboflavin  0.400
Sodium selenite  0.0173   Thiamine hydrochloride  4.000
AMINO ACIDS     Vitamin B12  0.013
Glycine 30.000   i-Inosito 7.200
L-Alanine  25.000   OTHERS  
L-Alanyl-L-Glutamine  868.000   D-Glucose  4500.000
L-Arginine hydrochloride  84.000   HEPES Buffer  5958.000
L-Asparagine  25.000   Phenol red sodium salt  15.000
L-Aspartic acid  30.000   Sodium pyruvate  110.000
L-Cysteine dihydrochloride  91.240      
L-Glutamic acid  75.000      
L-Histidine hydrochloride monohydrate  42.000      
L-Isoleucine  104.800      
L-Leucine  104.800      
L-Lysine hydrochloride  146.200      
L-Methionine  30.000      
L-Phenylalanine  66.000      
L-Proline  40.000      
L-Serine  42.000      
L-Threonine  95.200      
L-Tryptophan  16.000      
L-Tyrosine disodium salt  104.290      
L-Valine  93.600      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 280.00 -320.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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