HiGlutaXLTM Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine and Sodium bicarbonate, w/o HEPES buffer and Trace elements (Liquid)

Product#: HM-AL155G
$36.61
HM-AL155G
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HiGlutaXL™ Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture)
With L-Alanyl-L-Glutamine and 2.438gms per litre Sodium bicarbonate
Without HEPES buffer and Trace elements
1X Liquid Cell Culture Medium

Product Code: HM-AL155G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4ºC for longer periods.

AL155G is HiGlutaXL™ DMEM/Nutrient Mixture F-12 Ham with L-alanyl-L-glutamine and 2.438gms per litre sodium bicarbonate. It does not contain trace elements and HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  154.500   Choline chloride  8.980
Copper sulphate pentahydrate  0.0013   D-Biotin  0.0035
Disodium hydrogen phosphate  71.020   D-Ca-Pantothenate  2.240
Ferric nitrate nonahydrate  0.050   Folic acid  2.660
Ferrous sulphate heptahydrate  0.417   Niacinamide  2.020
Magnesium chloride hexahydrate  61.200   Pyridoxine hydrochloride  2.000
Magnesium sulphate anhydrous  48.840   Pyridoxine hydrochloride 0.031
Manganese sulphate  0.000151   Riboflavin  0.219
Potassium chloride  311.800    Thiamine hydrochloride  2.170
Sodium bicarbonate  2438.000   Vitamin B12  0.680
Sodium chloride  6996.000   myo-Inositol  12.600
Sodium dihydrogen phosphate  54.300   OTHERS  
Zinc sulphate heptahydrate  0.432   D-Glucose  3151.000
AMINO ACIDS     DL-Thioctic acid  0.105
Glycine 18.750   Linoleic acid  0.042
L-Alanine  4.450   Phenol red sodium salt  8.630
L-Arginine hydrochloride  147.500   Putrescine hydrochloride  0.081
L-Asparagine monohydrate  7.500   Sodium pyruvate  110.000
L-Aspartic acid  6.650   Thymidine  0.365
L-Cysteine dihydrochloride  17.560      
L-Cystine hydrochloride monohydrate  31.290      
L-Glutamic acid  7.350      
L-Alanyl-L-glutamine  543.000      
L-Histidine hydrochloride monohydrate  31.480      
L-Isoleucine  54.470      
L-Leucine  59.050      
L-Lysine hydrochloride  91.250      
L-Methionine  17.240      
L-Phenylalanine  35.480      
L-Proline  17.250      
L-Serine  26.250      
L-Threonine  53.450      
L-Tryptophan  9.020      
L-Tyrosine disodium salt  48.100      
L-Valine  52.850      


Quality Control:
 
  • Appearance: Red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 320.00 -360.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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