HiGlutaXLTM Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer (Liquid)

Product#: HM-AL127G
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture)
With L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements
Without HEPES buffer
1X Liquid Cell Culture Medium

Product Code: HM-AL127G

Product Description:

HiGlutaXL™ medium contains the stabilized dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL139G is HiGlutaXL™ DMEM/Nutrient Mixture F-12 Ham with L-alanyl-L-glutamine, sodium bicarbonate, 15mM HEPES buffer and trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Ingredients mg/L   Ingredients mg/L
Ammonium metavanadate  0.00058   Choline chloride  8.980
Ammonium molybdate tetrahydrate  0.00618   D-Biotin  0.0035
Copper sulphate pentahydrate  0.0013   D-Ca-Pantothenate  2.240
Disodium hydrogen phosphate  71.020   Folic acid  2.660
Ferric nitrate nonahydrate  0.050   Niacinamide  2.020
Ferrous sulphate heptahydrate  0.417   Pyridoxine hydrochloride  2.000
Magnesium chloride hexahydrate  61.200   Pyridoxine hydrochloride 0.031
Magnesium sulphate anhydrous  48.840   Riboflavin  0.219
Manganese sulphate  0.000151    Thiamine hydrochloride  2.170
Nickel chloride  0.00012   Vitamin B12  0.680
Sodium bicarbonate  1200.000   myo-Inositol  12.600
Potassium chloride  311.800   OTHERS  
Sodium chloride  6996.000   D-Glucose  3151.000
Sodium dihydrogen phosphate  54.300   DL-Thioctic acid  0.105
Sodium metasillicate nonahydrate  0.0142   HEPES buffer  3574.500
Sodium selenite  0.00519   Hypoxanthine  2.400
Stannous chloride dihydrate  0.00011   Linoleic acid  0.042
Zinc sulphate heptahydrate  0.432   Phenol red sodium salt  8.630
AMINO ACIDS     Putrescine hydrochloride  0.081
Glycine 18.750   Sodium pyruvate  110.000
L-Alanine  4.450   Thymidine 0.365
L-Arginine hydrochloride  147.500      
L-Asparagine monohydrate  7.500      
L-Aspartic acid  6.650      
L-Cysteine dihydrochloride  17.560      
L-Cystine hydrochloride monohydrate  31.290      
L-Glutamic acid  7.350      
L-Alanyl-L-glutamine  543.000      
L-Histidine hydrochloride monohydrate  31.480      
L-Isoleucine  54.470      
L-Leucine  59.050      
L-Lysine hydrochloride  91.250      
L-Methionine  17.240      
L-Phenylalanine  35.480      
L-Proline  17.250      
L-Serine  26.250      
L-Threonine  53.450      
L-Tryptophan  9.020      
L-Tyrosine disodium salt  48.100      
L-Valine  52.850      

Quality Control:
  • Appearance: Red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 290.00 -330.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 1EU/ml

Storage and shelf life:
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.

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