HiGlutaXLTM Dulbecco’s Modified Eagle Medium, High Glucose w/ L-Alanyl-L-Glutamine and Sodium bicarbonate w/o Sodium pyruvate (Liquid)

Product#: HM-AL066G
$32.62
HM-AL066G
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HiGlutaXL™ Dulbecco's Modified Eagle Medium, High Glucose
With L-Alanyl-L-Glutamine and Sodium bicarbonate
Without Sodium pyruvate
1X Liquid Cell Culture Medium

Product Code: HM-AL066G

Product Description:


HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of Lglutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4°C for longer periods.

AL066G is HiGlutaXL™ Dulbecco’s Modified Eagle Medium with L-alanyl-L-glutamine and sodium bicarbonate. It does not contain sodium pyruvate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


Composition:

Ingredients mg/L   Ingredients mg/L
INORGANIC SALTS   VITAMINS  
Calcium chloride dihydrate  265.000   Choline chloride  4.000
Ferric nitrate nonahydrate  0.100   D-Ca-Pantothenate  4.000
Magnesium sulphate anhydrous  97.720   Folic acid  4.000
Potassium chloride  400.000   Nicotinamide 4.000
Sodium bicarbonate  3700.000   Pyridoxal hydrochloride  4.000
Sodium chloride  6400.000   Riboflavin 0.400
Sodium dihydrogen phosphate anhydrous  109.000   Thiamine hydrochloride  4.000
AMINO ACIDS     i-Inositol  7.200
Glycine 30.000   OTHERS  
L-Arginine hydrochloride  84.000   D-Glucose  4500.000
L-Cysteine dihydrochloride  62.570   Phenol red sodium salt  15.900
L-Histidine hydrochloride  42.000      
L-Hydroxyproline  20.000      
L-Isoleucine  105.000      
L-Leucine  105.000      
L-Lysine hydrochloride  146.000      
L-Methionine  30.000      
L-Phenylalanine  66.000      
L-Serine  42.000      
L-Threonine  95.000      
L-Tryptophan  16.000      
L-Tyrosine disodium salt  103.790      
L-Valine  94.000      
L-alanyl-L-glutamine  868.000      


Quality Control:
 
  • Appearance: Orangish red colored, clear solution.
  • pH:7.00 -7.60
  • Osmolality in mOsm/Kg H2O: 330.00 -370.00
  • Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content: NMT 5EU/ml


Storage and shelf life:
 
  • Store at 2-8°C away from bright light.
  • Shelf life is 18 months.
  • Use before expiry date given on the product label.
 

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