Heparin sodium salt
Cell Culture Tested
Product Code: HM-TC138
Heparin is a linear polysaccharide consisting of repeating disaccharide units of L-iduronic acid or its epimer D-glucuronic acid and D-glucosamine, which is either N-acetylated or N-sulfated. Heparin is a highly sulfated form of heparan sulfate and belongs to glycosaminoglycan (GAG) group of sugars. Chemically it is acidic in nature. Single molecule of heparin carries 75-100 negative charges. As a result, heparin interacts with a large number of proteins and other basic molecules through ionic and hydrogen bonding interactions. It is involved in a wide range of fundamental biological processes, including proliferation, differentiation, tissue homeostasis, and viral pathogenesis.
- Cell proliferation: Heparin plays a bifunctional role in heterogenous primary cultures. It is used as an effective inhibitor of mesenchymal cell proliferation, whereas, it stimulates proliferation of primary epithelial cells. Hence heparin is extensively used in stem cell biology for study of stem cell growth kinetics.
- Serum free media: Heparin is used as a major component of serum free media for preventing cell aggregation. It promotes growth of human embryonic stem cells in a defined serum free medium by acting as a cofactor for FGF-2. It is required for wholesome growth of cells under serum free conditions.
- Coating of cell culture vessels: Heparin is used for coating surfaces of cell culture plates, flasks and dishes. Heparin interacts with cell adhesion molecules such as selectins and modulates cell adhesion process. Heparin-coated vessels are used in studying heparin-binding protein interactions.
- Cancer research: Heparin is used as an anti-cancer agent in in vitro cancer research. On entering the cells, heparin exerts cytotoxic action, causing cancer cell death by interfering with transcription factor activity. In addition to the above mentioned cell culture applications, heparin is extensively used as an anticoagulating agent in hematology.
The USP unit is a measure of the anticoagulant properties of a given heparin product as it acts on anti-thrombin III (AT-III).
Heparin is highly soluble in water (50mg/ml).Heparin solutions should be sterilized by filtering through a sterile membrane filter with a porosity of 0.22micron or less. Heparin solutions of heparin should not be autoclaved as autoclaving may cause cross-linking of the sugars and disruption of heparin structure. This may result in precipitation and loss of activity of heparin.
- Appearance: White to offwhite powder.
- Solubility: Clear colorless solution at 5gm in 100ml of water .
- pH of 1% solution in water: 5.00 -7.50
- Nitrogen: 1.3% -2.5%
- Heavy metals: NMT 0.003%
- Loss on drying: NMT 8%
- Potency in USP unit/mg: 140 UN/mg
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Cell Culture Test: Passes
Storage and Shelf Life:
- Store heparin powder at 2°-8°C away from bright light.
- Shelf life is 48 months.
- Heparin solutions remain stable for up to 2 year when stored at 2°-8°C. Being a polysaccharide, heparin is highly susceptible to microbial contamination. For long term storage, heparin solutions should be filter sterilized.
- Heparin solutions should not be stored at frozen temperatures.
- Use before expiry date given on the product label.