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iMatrix-511 (Laminin-511 E8)

Product Name

Size

Cat. No.

Storage

Maker

 Price (USD)

iMatrix-511

2x175μg

892001

4

NIP

USD 278.00

iMatrix-511

6x175μg

892002

4

NIP

USD 728.00

 

<Features>
* Simple Handling
* Can be used for various cells - especially iPS / ES cells
* No risk of contamination by xeno-components in your culture system.
* High cell viability and proliferation rate.
* 175 μg of iMatrix-511 can coat about 2 to 6 of 6 well plates.
* Feeder-free
* Easy to use

Product Information

User Manual


 


iMartix can be used for not only stem cells, but various cells, like epidermal cells, vascularendothelial cells.

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells and it can reduce the risk of the xeno-components contamination in the culture. Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temparature for 3 hours or at 4°C over night.

Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals,
and play an important role for cell adhesion and proliferation.

Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by
Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511". Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion. Although this is fragment, it has binding ability toalpha6beta1 integrin. According to Miyazaki's report ( Nat. Commun. 3, 1236 (2012)), Laminin-511 E8
could be used for cell culture of ES cells and iPS cells.

To date, mouse feeder cells or matrigel have been used for the culture of iPS cells and ES cells.
However, these cell culture substrates have a risk to bring xeno-components in the culture
condition so this was saidone of the problems for future medical and clinical application.



Fig. Human Epidemal Cells and Vascular Endothelical Cells cultured on iMartix-511. 
More efficient adhesion and extension can be seen when cultured on iMatrix-511.


This product is for research use only, not for diagnostic use.

<Reference>
Miner, JH.,et. al., Annu. Rev. Cell Dev. Biol. 20255284 (2004).
Ido, H.,et. al., J. Biol. Chem. 2791094610954 (2004).
Ido, H.,et. al., J. Biol. Chem. 2821114411154 (2007).
Taniguchi, Y.,et. al., J. Biol. Chem. 28478207831 (2009).
Miyazaki, T., et. al., Nat. Commun. 31236 (2012).

 

 

 

 

 

 

 

 

 

 

 

 

 

 
 
 
 
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