Maintain the morphology and proliferative ability of epithelium and endothelium tissues.
Tissues kept in ThelioKeep can be used for various applications.
Serum-free and protein-free formulation.
Free of bacteria, fungi and mycoplasma contamination.
Long shelf life. The product is stable for 1 years at 4°C after the date of manufacture.
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1. Dispense 0.5 ml of ThelioKeep and put it into the microtube containing
EGCG tablet provided. Pipette the entire solution back into the bottle
of ThelioKeep and mix well.
2. After the EGCG is completely dissolved, transfer a few milliliters of
solution to a centrifugation vial. Keep it cool at 4-10°C.
*Prepared ThelioKeep must be used as soon as possible. If storing for 1-2 weeks, separate into
aliquot and keep at -20°C. To prevent from loss of the activity, frequent freeze-thaw
method should be avoided.
3. Immerse the tissue isolated in the centrifugation vial with closed lid.
Store at 4°C.
4. Rince the living tissue with PBS, and then transfer it to the tissue
culture medium. Continue the further prodcedures according to appropriate
*For tissue culture procedures, place them under physiological conditions as soon as possible.
*For preparation of tissue sections, to preserve tissue morphology and retain the antogenicity
of the target molecules, fix the tissue with appropriate fixation solution immediately.
*For extraction of intracellular materials. wash the tissue with cooled PBS thoroughly.
*Institutes that are actively using our cryopreservation media.
- Stanford Medical School
- Harvard Medical School
- NCI, NIH
- Massachusetts General Hospital
- George Mason University