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StemCell Keep
StemCell Keep is a highly efficient Cryopreserving Solution for Human ES/iPS Cells without DMSO and Animal Protein.
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Cat. No.: VPL-A1
Size: 20ml
Price: USD200.00
Supplier: Funakoshi Co. Ltd.
Product data:  (4.1 MB)
This is available at NIH self-store refrigerator.
(NIH NSN#: 6550-00-L06-9024)
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Features
- Animal-derived Protein-free and DMSO-free formulation. No risk of differentiation effect by DMSO.
- Maintain ES and iPS cells with colony formation by vitrification.
- Maintain stem cell pluripotency after thawing.
- High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed
to cryopreserve ES and iPS cell colonies.
- Sufficient for 100 vials.
- Free of bacteria, fungi and mycoplasma contamination.
- Long shelf life. The product is stable for 2 years at 4°C after the date of manufacture.
Please click the following titles more detailed information.
Cryopreservation by Vitrification |
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Comparison Data |
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Protocol Outline |
The preliminary experiment is necessary before the actual experiment is carried out.
Cryopreserving
1. Place a dewar flask filled with liquid nitrogen in the clean bench.
2. Detach ES/iPS cell colonies with 0.25% trypsin / 1 mg/ml collagenase
IV / in PBS.
*One 60 mm dish of nearly confluent cells can be split into 1-5 vials.
3. Precipitate these cells by centrifugation and remove the supernatant. If
several vials are needed for cryopreservation, they are stored in ice.
4. Suspend the cells with 200 ul of StemCell Keep by pipetting. Close a lid
of the cryopreservation vial, and transfer it to the dewar flask within 1
minute.
*If the vitrification is successful, the solution should be removed transparent.
5. Transfer the vial to the liquid nitrogen chamber or -130°C deep freezer.
Thawing
1. Prepate each centrifugation vials each containing 9 ml of cell culture
medium warmed at 37°C in water bath.
*Thaw one tube at a time. Leave others frozen.
2. Put one cryopreservation vial containing cells into a dewar falsk filled with
liquid nitrogen, and place it on clean bench.
3. Add 1 ml of warmed cell culture medium into the cryopreserved vial immediately,
and then mix gently by pipetting.
4. Transfer the entire volume of diluted cells into the centrifugation vial, and
centrifuge to wash cells.
5. After transfer the cells to the feeder plate, continue the further culture
procedures according to standard protocols.
Precausion
Please prepare the medium and instruments for each experiment by reading the
instructions in advance. |
Experimental Results |
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CryoScarless DMSO-Free
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Cat. No.: CPL-A1
Size: 100ml
Price: USD147.00
Supplier: Funakoshi Co. Ltd.
Product data:
(1.85 MB)
This is available at NIH self-store refrigerator.
(NIH NSN#: 6550-00-L06-9025)
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Features
- Serum-free and DMSO-free formulation.
- High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins.
- Consistent and high cell viability after thawing. (>90% for most of the cell lines)
- Maintaining stem cell pluripotency after thawing.
- Free of bacteria, fungi and mycoplasma contamination.
- Long shelf life. The product is stable for 2 years at 4°C after the date of manufacture.
Please click the following titles more detailed information.
Comparison Data & Application |
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Protocol Outline |
1. Precipitate cultured cells by centrifugation and remove the supernatant.
2. Suspend the cells with CryoScarless DMSO-Free medium (1 ml for 5X105-106
cells). Dispense the cell suspension in 1 ml aliquots to cryopreservation vials.
3. Transfer the vials to -80°C deep freezer.
*For longe-term cryopreservation, thansfer the vials to liquid nitrogen storage tank.
4. Remove the cryopreserved cell from storage and quickly thaw in a 37°C shaking water bath.
Immediately dilute aznd gently mix each 1 ml of cells with 10 ml of appropriate cell
culture medium. |
Applications
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ThelioKeep
ThelioKeep is a serum-free and DMSO-free medium for preserving 1-2 weeks at 4°C.
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Cat. No.: TPO-A1
Size: 100ml
Price: USD147.00
Supplier: Funakoshi Co. Ltd.
Product data: (1.74 MB)
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Features
- Maintain the morphology and proliferative ability of epithelium and endothelium tissues.
- Tissues kept in ThelioKeep can be used for various applications.
- Serum-free and protein-free formulation.
- Free of bacteria, fungi and mycoplasma contamination.
- Long shelf life. The product is stable for 1 years at 4°C after the date of manufacture.
Please click the following titles more detailed information.
Comparison Data |
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Protocol Outline |
1. Dispense 0.5 ml of ThelioKeep and put it into the microtube containing
EGCG tablet provided. Pipette the entire solution back into the bottle
of ThelioKeep and mix well.
2. After the EGCG is completely dissolved, transfer a few milliliters of
solution to a centrifugation vial. Keep it cool at 4-10°C.
*Prepared ThelioKeep must be used as soon as possible. If storing for 1-2 weeks, separate into
aliquot and keep at -20°C. To prevent from loss of the activity, frequent freeze-thaw
method should be avoided.
3. Immerse the tissue isolated in the centrifugation vial with closed lid.
Store at 4°C.
4. Rince the living tissue with PBS, and then transfer it to the tissue
culture medium. Continue the further prodcedures according to appropriate
protocols.
*For tissue culture procedures, place them under physiological conditions as soon as possible.
*For preparation of tissue sections, to preserve tissue morphology and retain the antogenicity
of the target molecules, fix the tissue with appropriate fixation solution immediately.
*For extraction of intracellular materials. wash the tissue with cooled PBS thoroughly.
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*Institutes that are actively using our cryopreservation media.
- Stanford Medical School
- Harvard Medical School
- NCI, NIH
- Massachusetts General Hospital
- George Mason University
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